Data/hora: 17/05/2024 12:41:25

Biblioteca(s): 	Embrapa Recursos Genéticos e Biotecnologia; Embrapa Rondônia.	
Data corrente: 	05/06/2020	
Data da última atualização: 	15/09/2021	
Autoria: 	RODRIGUES, S. A. D.; PONTELO, T. P.; KUSSANO, N. R.; KAWAMOTO, T. S.; LEME, L. O.; CAIXETA, F. M. C.; PFEIFER, L. F. M.; FRANCO, M. M.; DODE, M. A. N.	
Afiliação: 	SARAH A. D. RODRIGUES, Universidade de Brasília; THAIS P. PONTELO, Universidade Federal de Lavras; NAYARA R. KUSSANO, Universidade de Brasília; TAYNAN S. KAWAMOTO, Universidade Federal de Uberlândia; LIGIANE O. LEME, Universidade Federal do Espírito Santo; FELIPPE M. C. CAIXETA, Universidade de Brasília; LUIZ FRANCISCO MACHADO PFEIFER, CPAF-RO; MAURICIO MACHAIM FRANCO, Cenargen; MARGOT ALVES NUNES DODE, Cenargen.	
Título: 	Effects of Prostaglandins E2 and F2alpha on the in vitro maturation of bovine oocytes.	
Ano de publicação: 	2020	
Fonte/Imprenta: 	Domestic Animal Endocrinology, v. 72, article 106447, 2020.	
ISSN: 	0739-7240	
DOI: 	https://doi.org/10.1016/j.domaniend.2020.106447	
Idioma: 	Inglês	
Conteúdo: 	We aimed to elucidate the effects of PGE2 and PGF2a on the in vitro maturation (IVM) of bovine oocytes. First, cumulus-oocyte complexes were matured in the media supplemented with or without PGE2, PGF2a, or PGE2 plus PGF2a for the final 24, 12, or 6 h of culture. Then, the cumulus-oocyte complexes were matured in the absence or presence of a PG endoperoxide synthase 2 (PTGS2) enzyme inhibitor (NS398) supplemented with PGE2, PGF2a, or PGE2 plus PGF2a. Finally, the expression of genes associated with PGs activity in cumulus cells (PTGS2, PG E-synthase-1 [PTGES1], and aldo-keto reductase 1 [AKR1B1]) or oocytes (receptors for PGE2 [PTGER2] and PGF2a [PTGFR]) of different competencies was quantified. Supplementation of the IVM medium with PGs did not improve in vitro embryo production or embryo quality (P > 0.05). During maturation, the relative abundance of PTGS2 transcripts increased (P < 0.05) only in the less-competent group, whereas those of PTGES1 increased in the less-competent and in the more-competent groups. Conversely, AKR1B1 expression decreased only in the less-competent group (P < 0.05). Receptors for the PGE2 and PGF2a genes were very low or undetectable in oocytes. In conclusion, PGE2 and PGF2a are not recommended for media supplementation during maturation because they have no effect on embryo development. Although genes related to PGs activity are differentially expressed in cumulus cells of cumulus-oocyte complexes of different competence during maturation, the expression of PGE2 and PGF2a receptor genes was either not detectable or was detected at low levels in oocytes.	
Palavras-Chave: 	E2; Embryonic development; F2a; Maturation; Oócito; Oocyte competence.	
Thesagro: 	Bovinocultura; Cultura In Vitro; Embriogénese; Maturação; Ovulo; Prostaglandina; Reprodução Animal.	
Thesaurus Nal: 	Animal reproduction; Cattle; Embryogenesis; Gene expression; In vitro studies; Oocytes; Prostaglandins.	
Categoria do assunto: 	--
K Ciência Florestal e Produtos de Origem Vegetal	
URL: 	http://ainfo.cnptia.embrapa.br/digital/bitstream/item/219006/1/cpafro-18481.pdf	
Registro original: 	Embrapa Rondônia (CPAF-RO)	
Exemplares: 	CENARGEN - (38188-1) - UPC - AP - DD -  -  -  - DIS
CPAF-RO - (18481-1) - UPC - AP - DD -  -  -  - DIS